tag:blogger.com,1999:blog-28479014822849134232024-02-07T20:22:00.646-08:00Freeze Substitution in hours, not daysTotalTemp Technologieshttp://www.blogger.com/profile/09999016708374087367noreply@blogger.comBlogger2125tag:blogger.com,1999:blog-2847901482284913423.post-55541520341912585892013-07-26T12:32:00.003-07:002013-07-26T12:32:36.686-07:00
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<b><span style="color: blue; font-family: "Arial Rounded MT Bold","sans-serif"; font-size: 12pt; mso-ligatures: none;"><span style="mso-spacerun: yes;"> </span>The <i>Next
Generation </i>Thermal Test Performance<o:p></o:p></span></b></h2>
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<b><span style="color: blue; font-family: "Arial Rounded MT Bold","sans-serif"; mso-contextual-alternates: yes; mso-font-kerning: 14.0pt;"><span style="mso-spacerun: yes;"> </span>A <i>New Generation </i>of Excellent Customer
Service<i><o:p></o:p></i></span></b></div>
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<b><u><span style="font-family: "Arial","sans-serif"; font-size: 18pt;">TOTALTEMP CRYO
MICROWAVE SAMPLE PROCESSOR <o:p></o:p></span></u></b></div>
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<b><span style="font-family: "Arial","sans-serif"; font-size: 14pt;">Microwave-Assisted
Freeze Substitution of Biological and Biomedical Samples </span></b><span style="font-family: "Arial","sans-serif"; font-size: 14pt;"><o:p></o:p></span></div>
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<b style="mso-bidi-font-weight: normal;"><span style="font-family: "Arial","sans-serif"; font-size: 16pt;">Cryo
Microwave Sample Processor with the TotalTemp Thermal Platform System specially
engineered to operate inside of the Ted Pella BioWave Microwave Oven<span style="mso-bidi-font-weight: bold;"><o:p></o:p></span></span></b></div>
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<b><span style="font-family: "Arial","sans-serif"; font-size: 16pt;">Description of Process:</span></b><span style="font-family: "Arial","sans-serif"; font-size: 16pt;"><o:p></o:p></span></div>
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<span style="font-family: "Arial","sans-serif"; font-size: 16pt;">Freeze substitution fixation (<b style="mso-bidi-font-weight: normal;">FS</b>) of hydrated samples frozen in vitreous ice provides exceptional
preservation of structure for light and electron microscopy, and enables
immunological detection of thermo-labile antigens that otherwise are
damaged/destroyed by processing at ambient or elevated temperatures.<span style="mso-spacerun: yes;"> </span>Its use as a research tool or in clinical
pathology has, however, been limited by the relatively lengthy periods required
for passive diffusion of fixatives and organic solvents into the frozen
hydrated material.<o:p></o:p></span></div>
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<span style="font-family: "Arial","sans-serif"; font-size: 16pt;">The invention utilizes
controlled microwave (<b style="mso-bidi-font-weight: normal;">MW</b>)
irradiation to accelerate the FS process; and comprises systems, devices and
methods for microwave-assisted processing of samples under cryo-conditions,
typically -80°C.<span style="mso-spacerun: yes;"> </span>The entire MWFS
procedure is now accomplished in less than 4 hours as compared to the
approximately 4-5 days previously required for FS process.<o:p></o:p></span></div>
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<span style="font-family: "Arial","sans-serif"; font-size: 16pt;">The Cryo Microwave Sample Processor System combines two
specific technologies, controlled microwave irradiation and temperature
cycling.<span style="mso-spacerun: yes;"> </span>The controlled temperature
cycling device, a Thermal Platform (a.k.a. Hot/Cold Plate) was specially
engineered by TotalTemp using some alternative nonmetallic materials to allow
it to operate inside of a microwave environment.<span style="mso-spacerun: yes;"> </span>The Thermal Platform is cooled with Liquid Nitrogen
(L-N</span><sup><span style="font-family: "Arial","sans-serif"; font-size: 18pt;">2</span></sup><span style="font-family: "Arial","sans-serif"; font-size: 16pt;">) giving it a
precisely controlled temperature range of -100°C to +200°C.<span style="mso-spacerun: yes;"> </span><o:p></o:p></span></div>
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<span style="font-family: "Arial","sans-serif"; font-size: 16pt;">The microwave oven was provided to TotalTemp by the
customer to be modified for the installation of a bulkhead interface plate to
allow the input of AC power, L-N</span><sup><span style="font-family: "Arial","sans-serif"; font-size: 18pt;">2</span></sup><span style="font-family: "Arial","sans-serif"; font-size: 16pt;"> and a nitrogen gas purge to prevent
excessive condensation inside the oven while running the platform at cryo
temperatures.<span style="mso-spacerun: yes;"> </span>The nitrogen gas purge is
directed inside of a clear polycarbonate cover that rests over the platform
surface.<span style="mso-spacerun: yes;"> </span>TotalTemp machined a custom
designed adapter plate with recessed pockets to support vials and petri dishes
for thermal conduction during the FS process.<span style="mso-spacerun: yes;">
</span>The Microwave Oven is a Ted Pella Biowave Model 3451 which has special Magnetron
for continuous power from 80-750W, not pulsing power like a standard kitchen
oven.<o:p></o:p></span></div>
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<span style="font-family: "Arial","sans-serif"; font-size: 16pt;"><o:p> </o:p></span></div>
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<b><span style="font-family: "Arial","sans-serif"; font-size: 16pt;">Applications:</span></b><span style="font-family: "Arial","sans-serif"; font-size: 16pt;"><o:p></o:p></span></div>
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<li class="MsoNormal" style="margin: 0in 0in 0pt; mso-list: l0 level1 lfo1; tab-stops: list .5in; text-align: justify;"><span style="font-family: "Arial","sans-serif"; font-size: 16pt;">Provides
superior preservation and rapid turnaround in research and high throughput
clinical laboratory settings<o:p></o:p></span></li>
<li class="MsoNormal" style="margin: 0in 0in 0pt; mso-list: l0 level1 lfo1; tab-stops: list .5in; text-align: justify;"><span style="font-family: "Arial","sans-serif"; font-size: 16pt;">Applicable
to a broad range of biological samples, hydrogels, and other hydrated
materials<o:p></o:p></span></li>
<li class="MsoNormal" style="margin: 0in 0in 0pt; mso-list: l0 level1 lfo1; tab-stops: list .5in; text-align: justify;"><span style="font-family: "Arial","sans-serif"; font-size: 16pt;">Processing
for light and electron microscopy<o:p></o:p></span></li>
<li class="MsoNormal" style="margin: 0in 0in 0pt; mso-list: l0 level1 lfo1; tab-stops: list .5in; text-align: justify;"><span style="font-family: "Arial","sans-serif"; font-size: 16pt;">Low-temperature
synthetic and analytical chemistry<o:p></o:p></span></li>
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<b><span style="font-family: "Arial","sans-serif"; font-size: 16pt;">Advantages:</span></b><span style="font-family: "Arial","sans-serif"; font-size: 16pt;"><o:p></o:p></span></div>
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<li class="MsoNormal" style="margin: 0in 0in 0pt; mso-list: l1 level1 lfo2; tab-stops: list .5in; text-align: justify;"><span style="font-family: "Arial","sans-serif"; font-size: 16pt;">Reduces
processing periods from days to hours<o:p></o:p></span></li>
<li class="MsoNormal" style="margin: 0in 0in 0pt; mso-list: l1 level1 lfo2; tab-stops: list .5in; text-align: justify;"><span style="font-family: "Arial","sans-serif"; font-size: 16pt;">Improves
preservation, approaching native state<o:p></o:p></span></li>
<li class="MsoNormal" style="margin: 0in 0in 0pt; mso-list: l1 level1 lfo2; tab-stops: list .5in; text-align: justify;"><span style="font-family: "Arial","sans-serif"; font-size: 16pt;">Enables
uncomplicated, programmable operation<o:p></o:p></span></li>
<li class="MsoNormal" style="margin: 0in 0in 0pt; mso-list: l1 level1 lfo2; tab-stops: list .5in; text-align: justify;"><span style="font-family: "Arial","sans-serif"; font-size: 16pt;">Provides
excellent reproducibility with full logging and network printing
capability<o:p></o:p></span></li>
<li class="MsoNormal" style="margin: 0in 0in 0pt; mso-list: l1 level1 lfo2; tab-stops: list .5in; text-align: justify;"><span style="font-family: "Arial","sans-serif"; font-size: 16pt;">Barcode
scanning option available<o:p></o:p></span></li>
</ul>
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<b style="mso-bidi-font-weight: normal;"><span style="font-family: "Arial","sans-serif"; font-size: 16pt;">Custom
Configured Adapter Plate<o:p></o:p></span></b></div>
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<span style="font-family: "Arial","sans-serif"; font-size: 14pt; mso-no-proof: yes;">TotalTemp Thermal Platform
outside of oven showing custom Adapter Plate<o:p></o:p></span></div>
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<span style="font-family: "Arial","sans-serif"; font-size: 14pt; mso-no-proof: yes;">designed to support specific
vials and petri dishes for tissue sample preparation<o:p></o:p></span></div>
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<b style="mso-bidi-font-weight: normal;"><span style="font-family: "Arial","sans-serif"; font-size: 16pt;">Freeze
Substitution Time and Temperature Chart<o:p></o:p></span></b></div>
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<span style="font-family: "Arial","sans-serif"; font-size: 14pt;">Time
Comparison Chart of sample preparation while under microwave irradiation (red line)
vs. sample preparation without microwave irradiation (purple line).<span style="mso-spacerun: yes;"> </span>Note <o:p></o:p></span></div>
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<span style="font-family: "Arial","sans-serif"; font-size: 14pt;">the
tremendous time difference on the “Minutes” scale.<o:p></o:p></span></div>
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<b style="mso-bidi-font-weight: normal;"><span style="font-family: "Arial","sans-serif"; font-size: 16pt;">Transmission Electron Microscope images of Bacillus Subtilis,
using ambient and cryo-preparative methods.<span style="mso-spacerun: yes;">
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<span style="font-family: "Arial","sans-serif"; font-size: 14pt;">Mid-log
phase cultures were prepared by conventional chemical fixation (A), MW-assisted
chemical fixation (B), traditional FS (C), or by rapid FS without (D) or with
MW irradiation (E-F).<span style="mso-spacerun: yes;"> </span>Inserts show
detailed cell wall structure at a magnification equivalent to that in panel
D.<span style="mso-spacerun: yes;"> </span>Samples processed rapidly by FS
without MW irradiation exhibited poor preservation (D).<span style="mso-spacerun: yes;"> </span>Samples processed by MWFS exhibited
structures such as membranes, ribosomes, and cell wall material that were
obscured or poorly discernible by other techniques.<span style="mso-spacerun: yes;"> </span>Bars: 50 nm.<o:p></o:p></span></div>
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TotalTemp Technologieshttp://www.blogger.com/profile/09999016708374087367noreply@blogger.com0tag:blogger.com,1999:blog-2847901482284913423.post-42259508652688976272013-06-11T10:33:00.000-07:002013-06-11T10:33:22.389-07:00<div style="text-align: center;">
Bio processor system dramatically cuts the time required for Freeze Substitution of tissue samples</div>
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